| SPEEDTOOLS DNA and RNA Kits | |||||||||||||||||||||||||||||||||||
| SPEEDTOOLS DNA and RNA Extraction Kits | |||||||||||||||||||||||||||||||||||
| The SPEEDTOOLS DNA (genomic, blood) Kits are designed for the rapid isolation of highly pure genomic DNA from whole blood, serum, plasma, or other body fluids. It is also possible to purify viral DNA (e.g. HBV) from blood samples. As viral DNA co-purifies with cellular DNA, we recommend usage of cell-free sample (serum or plasma) to prepare pure viral DNA. · Blood treated either with EDTA, citrate, or heparin can be used. If leukocyte rich materials like buffy coat are used, apply smaller volumes and dilute the samples with sterile PBS (dissolve 8 g NaCl, 0.2 g KCl, 1.44 g Na2HPO4, and 0.24 g KH2PO4 in 800 ml H2O. Adjust pH to 7.4 with HCl. Add H2O to 1 liter)· The kits allow purification of highly pure genomic DNA with an A260/280-ratio between 1.60 and 1.90 and a typical concentration of 40 - 60 ng per µl for the SPEEDTOOLS DNA Blood kit.· The obtained DNA is ready to use for subsequent reactions like PCR, Southern blotting, or any kind of enzymatic reactions. SPEEDTOOLS TISSUE DNA Kit is intended for extraction and purification of DNA from tissues. This kit is optimal for extraction of Mycobacterium tuberculosis DNA. SPEEDTOOLS MYCOBACTERIA DNA Kit with CE-MARK! SPEEDTOOLS 96-well DNA and TISSUE DNA Extraction Kits for use with your automatic Corbett X-Tractor! SPEEDTOOLS PLANT and FOOD DNA Kit for extraction and purification of genomic DNA from plant and processed food without delay and problems. The ideal combination with your BIOGENICS and BIOFOOD kits. SPEEDTOOLS RNA Virus Extraction Kit is designed for the rapid preparation of highly pure viral nucleic acids (e.g. HCV, HIV, CMV) from fluid biological samples e.g. plasma, serum, urine but not blood (see remarks in section 2.1).· No cross contamination due to closed systems.· The SPEEDTOOLS RNA Virus kit works with 150 µl serum.The prepared nucleic acids are suitable for applications like automated fluorescent DNA sequencing, RT-PCR*, or any kind of enzymatic manipulation. The detection limit for certain viruses depends on individual detection procedures e.g. in-house nested (RT-) PCR. We highly recommend the use of internal (low-copy) standards as well as positive and negative controls in order to monitor the purification, amplification and detection processes. | |||||||||||||||||||||||||||||||||||
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