Plasmid pKT1 (1 µg at 100 ng / µl) - Cat. No. 31.017

Plasmid pKT1 is a pUC-derived plasmid containing the kat cassette. This cassette is expressed under the control of a promoter active in Escherichia coli and Thermus thermophilus. The cassette codifies for kanamycine resistance (30 µg / ml). The cassette can be easily extracted by using the restriction sites included in its flanking polylinker.

(Lasa et al (1992). Mol.Microbiol., 11: 1555-1564). Patent number P9301522.

Plasmid pMK18 (1 µg at 100 ng / µl) - Cat. No. 31.018

Plasmid pMK18 has been obtained from Escherichia coli and Thermus thermophilus sequences, contaning ori sites. Therefore, it is a shuttle vector, that can be used for thermostabilisation studies involving transformation of both organisms. The plasmid contains the kat cassette (see pKT1) as a selectable marker.

(De Grado, M. Ph.D. Thesis, UAM, Madrid, Spain). Patent number P9301522.

Plasmid pMKE1 is a pUC-derived plasmid containing the kat cassette. The cassette codifies for kanamycine resistance (30 µg / ml). The cassette can be easily extracted by using the restriction sites included in its flanking polylinker. Also, the plasmid includes the Pnar promoter, for nitrate-induced expression. Please note that this plasmid has to be used with a special Thermus strain (HB27 :: nar), available in our catalogue (Cat. No. 31.121).

Plasmid pMKE2 is a pUC-derived plasmid containing the kat cassette. The cassette codifies for kanamycine resistance (30 µg / ml). The cassette can be easily extracted by using the restriction sites included in its flanking polylinker. Also, the plasmid includes the Pnar promoter, for nitrate-induced expression. Please note that this plasmid has to be used with a special Thermus strain (HB27 :: nar), available in our catalogue (Cat. No. 31.121).