- Cloning and expression system for Thermus thermophilus.
- Applications of the kit include: cloning and expression of thermophilic
proteins in a host system, thermostabilistation of mesophilic proteins
and direct selection of thermostable mutants in a thermophilic organism,
overexpression of mesophilic proteins that cannot be expressed by the
usual means.
- High efficiency of pMKE1 or pMKE2 vectors in transformation
of Thermus thermophilus HB27::nar strain.
- Use of Thermus HB27::nar competent cells optimised for transformation
with exogenous plasmid DNA.
- Fast screening of transformants due to kanamycine-resistance encoded
by pMKE1 and pMKE2 vectors.
- No recombination processes inThermus.
- Exclusive incubation chamber optimised for growth of bacteria at high
temperatures.
- Optimised protocol and media for efficient transformation.
- User's manual (pdf)
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